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Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch

Brassard Nicole, Brissette Line, Lord Daniel et Laliberté Sylvie. (1996). Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch. Plant Cell, Tissue and Organ Culture, 44, p. 37-44.

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Résumé

Factors were defined for elongation, rooting and acclimatization of micropropagated shoots of Larix x eurolepis Henry initiated from short shoot buds of plagiotropic stecklings serially propagated for 9 years from an 8-year-old tree. Initiation and multiplication were on Schenk and Hildebrandt (SH) medium supplemented with 5 μM 6-benzyladenine (BA) and 1 μM indole-butyric acid (IBA). Stem elongation was obtained in 36% of the shoots on SH medium containing 0.5 μM BA and 63% of the remaining non-elongated shoots initiated stem elongation after transfer on SH medium devoid of growth regulators. Rooting involved 2 steps: root induction on Campbell and Durzan mineral salts and Murashige and Skoog organic elements, both half-strength (CD-MS/2), supplemented with 1 μM of both naphthaleneacetic acid (NAA) and IBA, and root elongation following transfer to CD-MS/2 medium devoid of growth regulators. Repeating this 2-step sequence yielded up to 67% rooted shoots. Acclimatization of plantlets ranged from 83% to 100%. Over 300 plants were transferred to the greenhouse; some showed plagiotropic growth.

Type de document:Article publié dans une revue avec comité d'évaluation
Volume:44
Pages:p. 37-44
Version évaluée par les pairs:Oui
Date:1996
Sujets:Sciences naturelles et génie > Sciences naturelles > Biologie et autres sciences connexes
Département, module, service et unité de recherche:Départements et modules > Département des sciences fondamentales > Modules des sciences fondamentales
Mots-clés:acclimatization, conifer, Larix x eurolepis, mature material, plagiotropism, rooting, stem elongation
Déposé le:15 janv. 2016 01:40
Dernière modification:15 janv. 2016 01:40
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