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Huntingtin Interacting Protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain

Legendre-Guillemin Valérie, Metzler Martina, Lemaire Jean-François, Philie Jacynthe, Gan Lu, Hayden Michael R. et McPherson Peter S.. (2005). Huntingtin Interacting Protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain. The Journal of Biological Chemistry, 280, (7), p. 6101-6108.

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URL officielle: http://dx.doi.org/doi:10.1074/jbc.M408430200

Résumé

Huntingtin interacting protein 1 (HIP1) is a component of clathrin coats. We previously demonstrated that HIP1 promotes clathrin assembly through its central helical domain, which binds directly to clathrin light chains (CLCs). To better understand the relationship between CLC binding and clathrin assembly we sought to dissect this interaction. Using C-terminal deletion constructs of the HIP1 helical domain, we identified a region between residues 450 and 456 that is required for CLC binding. Within this region, point mutations showed the importance of residues Leu-451, Leu-452, and Arg-453. Mutants that fail to bind CLC are unable to promote clathrin assembly in vitro but still mediate HIP1 homodimerization and heterodimerization with the family member HIP12/HIP1R. Moreover, HIP1 binding to CLC is necessary for HIP1 targeting to clathrin-coated pits and clathrin-coated vesicles. Interestingly, HIP1 binds to a highly conserved region of CLC previously demonstrated to regulate clathrin assembly. These results suggest a role for HIP1/CLC interactions in the regulation of clathrin assembly.

Type de document:Article publié dans une revue avec comité d'évaluation
ISSN:00219258 (ISSN)
Volume:280
Numéro:7
Pages:p. 6101-6108
Version évaluée par les pairs:Oui
Date:2005
Sujets:Sciences de la santé > Sciences médicales > Biochimie
Sciences de la santé > Sciences médicales > Biologie cellulaire
Sciences de la santé > Sciences médicales > Biologie moléculaire
Département, module, service et unité de recherche:Départements et modules > Département des sciences fondamentales
Mots-clés:Huntingtin interacting protein 1, HIP1, clathrin, endocytosis, binding sites, Huntington disease, protein
Informations complémentaires:This research was originally published in The Journal of Biological Chemistry. Legendre-Guillemin V, Metzler M, Lemaire JF, Philie J, Gan L, Hayden MR et McPherson PS. Huntingtin Interacting Protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain. Journal of Biological Chemistry. 2005; 280 : 6101-6108. © the American Society for Biochemistry and Molecular Biology
Déposé le:01 juin 2016 17:33
Dernière modification:05 déc. 2016 20:47
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