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HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2

Metzler Martina, Legendre-Guillemin Valérie, Gan Lu, Chopra Vikramjit, Kwok Anita, McPherson Peter S. et Hayden Michael R.. (2001). HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2. The Journal of Biological Chemistry, 276, (42), p. 39271-39276.

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URL officielle: http://dx.doi.org/doi:10.1074/jbc.C100401200

Résumé

Polyglutamine expansion in huntingtin is the underlying mutation leading to neurodegeneration in Huntington disease. This mutation influences the interaction of huntingtin with different proteins, including huntingtin-interacting protein I (HIP(), in which affinity to bind to mutant huntingtin is profoundly reduced. Here we demonstrate that HIP( colocalizes with markers of clathrin-mediated endocytosis in neuronal cells and is highly enriched on clathrin-coated vesicles (CCVs) purified from brain homogenates. HIP( binds to the clathrin adaptor protein 2 (AP2) and the terminal domain of the clathrin heavy chain, predominantly through a small fragment encompassing amino acids 276-335. This region, which contains consensus clathrin- and AP2-binding sites, functions in conjunction with the coiled-coil domain to target HIP( to CCVs. Expression of various HIP1 fragments leads to a potent block of clathrin-mediated endocytosis. Our findings demonstrate that HIP1 is a novel component of the endocytic machinery.

Type de document:Article publié dans une revue avec comité d'évaluation
ISSN:0021-9258
Volume:276
Numéro:42
Pages:p. 39271-39276
Version évaluée par les pairs:Oui
Date:2001
Sujets:Sciences de la santé > Sciences médicales > Biochimie
Sciences de la santé > Sciences médicales > Biologie cellulaire
Sciences de la santé > Sciences médicales > Biologie moléculaire
Département, module, service et unité de recherche:Départements et modules > Département des sciences fondamentales
Mots-clés:Huntington disease, HIP1, huntingtin-interacting protein 1, mutation, clathrin, endocytosis
Informations complémentaires:This research was originally published in The Journal of Biological Chemistry. Metzler M, Legendre-Guillemin V, Gan L, Chopra V, Kwok A, McPherson PS et Hayden MR. HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2. Journal of Biological Chemistry. 2001; 276 : 39271-39276. © the American Society for Biochemistry and Molecular Biology.
Déposé le:01 juin 2016 14:48
Dernière modification:05 déc. 2016 20:49
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