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Isoprene emission by poplar is not important for the feeding behaviour of poplar leaf beetles

Müller Anna, Kaling Moritz, Faubert Patrick, Gort Gerrit, Smid Hans M, Van Loon Joop JA, Dicke Marcel, Kanawati Basem, Schmitt-Kopplin Philippe, Polle Andrea, Schnitzler Jörg-Peter et Rosenkranz Maaria. (2015). Isoprene emission by poplar is not important for the feeding behaviour of poplar leaf beetles. BMC Plant Biology, 15, (165), p. 1-16.

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URL officielle: http://dx.doi.org/doi:10.1186/s12870-015-0542-1

Résumé

Background : Chrysomela populi (poplar leaf beetle) is a common herbivore in poplar plantations whose infestation causes major economic losses. Because plant volatiles act as infochemicals, we tested whether isoprene, the main volatile organic compound (VOC) produced by poplars (Populus x canescens), affects the performance of C. populi employing isoprene emitting (IE) and transgenic isoprene non-emitting (NE) plants. Our hypothesis was that isoprene is sensed and affects beetle orientation or that the lack of isoprene affects plant VOC profiles and metabolome with consequences for C. populi feeding.

Results : Electroantennographic analysis revealed that C. populi can detect higher terpenes, but not isoprene. In accordance to the inability to detect isoprene, C. populi showed no clear preference for IE or NE poplar genotypes in the choice experiments, however, the beetles consumed a little bit less leaf mass and laid fewer eggs on NE poplar trees in field experiments. Slight differences in the profiles of volatile terpenoids between IE and NE genotypes were detected by gas chromatography - mass spectrometry. Non-targeted metabolomics analysis by Fourier Transform Ion Cyclotron Resonance Mass Spectrometer revealed genotype-, time- and herbivore feeding-dependent metabolic changes both in the infested and adjacent undamaged leaves under field conditions.

Conclusions : We show for the first time that C. populi is unable to sense isoprene. The detected minor differences in insect feeding in choice experiments and field bioassays may be related to the revealed changes in leaf volatile emission and metabolite composition between the IE and NE poplars. Overall our results indicate that lacking isoprene emission is of minor importance for C. populi herbivory under natural conditions, and that the lack of isoprene is not expected to change the economic losses in poplar plantations caused by C. populi infestation.

Type de document:Article publié dans une revue avec comité d'évaluation
ISSN:1471-2229
Volume:15
Numéro:165
Pages:p. 1-16
Version évaluée par les pairs:Oui
Date:2015
Identifiant unique:10.1186/s12870-015-0542-1
Sujets:Sciences naturelles et génie > Sciences naturelles > Biologie et autres sciences connexes
Sciences naturelles et génie > Sciences naturelles > Chimie
Département, module, service et unité de recherche:Départements et modules > Département des sciences fondamentales
Mots-clés:Chrysomela populi, Volatile organic compounds, VOC, Isoprene, Isoprenoids, Terpene, Electroantennography, Beetle behaviour, Populus x canescens, Metabolomics, composés volatils organiques, isoprénoïdes, électroantennographie, chrysomètre du peuplier, poplar leaf beetles, métabolomique
Déposé le:21 janv. 2021 01:07
Dernière modification:21 janv. 2021 01:07
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