Metzler Martina, Legendre-Guillemin Valérie, Gan Lu, Chopra Vikramjit, Kwok Anita, McPherson Peter S. et Hayden Michael R.. (2001). HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2. The Journal of Biological Chemistry, 276, (42), p. 39271-39276.
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URL officielle: http://dx.doi.org/doi:10.1074/jbc.C100401200
Résumé
Polyglutamine expansion in huntingtin is the underlying mutation leading to neurodegeneration in Huntington disease. This mutation influences the interaction of huntingtin with different proteins, including huntingtin-interacting protein I (HIP(), in which affinity to bind to mutant huntingtin is profoundly reduced. Here we demonstrate that HIP( colocalizes with markers of clathrin-mediated endocytosis in neuronal cells and is highly enriched on clathrin-coated vesicles (CCVs) purified from brain homogenates. HIP( binds to the clathrin adaptor protein 2 (AP2) and the terminal domain of the clathrin heavy chain, predominantly through a small fragment encompassing amino acids 276-335. This region, which contains consensus clathrin- and AP2-binding sites, functions in conjunction with the coiled-coil domain to target HIP( to CCVs. Expression of various HIP1 fragments leads to a potent block of clathrin-mediated endocytosis. Our findings demonstrate that HIP1 is a novel component of the endocytic machinery.
| Type de document: | Article publié dans une revue avec comité d'évaluation |
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| ISSN: | 0021-9258 |
| Volume: | 276 |
| Numéro: | 42 |
| Pages: | p. 39271-39276 |
| Version évaluée par les pairs: | Oui |
| Date: | 2001 |
| Sujets: | Sciences de la santé > Sciences médicales > Biochimie Sciences de la santé > Sciences médicales > Biologie cellulaire Sciences de la santé > Sciences médicales > Biologie moléculaire |
| Département, module, service et unité de recherche: | Départements et modules > Département des sciences fondamentales |
| Mots-clés: | Huntington disease, HIP1, huntingtin-interacting protein 1, mutation, clathrin, endocytosis |
| Informations complémentaires: | This research was originally published in The Journal of Biological Chemistry. Metzler M, Legendre-Guillemin V, Gan L, Chopra V, Kwok A, McPherson PS et Hayden MR. HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2. Journal of Biological Chemistry. 2001; 276 : 39271-39276. © the American Society for Biochemistry and Molecular Biology. |
| Déposé le: | 01 juin 2016 14:48 |
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| Dernière modification: | 05 déc. 2016 20:49 |
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