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Cytotoxic, anti-inflammatory and antioxidant activities of four different extracts of Galega officinalis L (Goat’s rue)

Karakas Fatma Pehlivan, Turker Arzu Ucar, Karakas Alper et Mshvildadze Vakhtang. (2016). Cytotoxic, anti-inflammatory and antioxidant activities of four different extracts of Galega officinalis L (Goat’s rue). Tropical Journal of Pharmaceutical Research, 15, (4), p. 751-757.

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URL officielle: http://dx.doi.org/doi:10.4314/tjpr.v15i4.12

Résumé

Purpose: To evaluate the cytotoxic, anti-inflammatory and antioxidant activities of four different solvent extracts obtained from the aerial parts of Galega officinalis L.

Methods: The hexane, DCM, methanol and water extracts of G. officinalis were successively obtained by soxhlet extraction method. The cytotoxic activity of the extracts was assessed against human lung carcinoma (A-549), human colorectal adenocarcinoma (HT-29), human brain glioblastoma (U-87), and colon adenocarcinoma (DLD-1) by Resazurine test. The antioxidant activity of extracts were determined by Folin-Ciocalteau, oxygen radical absorbing capacity (ORAC), and 2’.7’-dichlorofluorescin-diacetate (DCFH-DA) cell-based assay while their anti-inflammatory activity was determined by nitric oxide (NO) assay.

Results: DCM extract showed strong cytotoxic activity against lung adenocarcinoma and brain glioblastoma cell lines, with IC50 (concentration inhibiting 50 % of cell growth) values of 11 ± 0.4 and 16 ± 3 μg/mL, respectively. The hexane extract showed moderate anticancer activity against the same cell lines (59 ± 13 and 63 ± 16 μg/mL, respectively). DCM extract also showed significant anti-inflammatory activity, inhibiting NO release by 86.7 % at 40 μg/mL in lipopolysaccharide (LPS) - stimulated murine RAW 264.7 macrophages. Of all test extracts, the methanol extract of G. officinalis showed the highest antioxidant activity with 2.33 ± 0.09 μmol Trolox/mg, 7.10 ± 0.9 g tannic acid equivalent (TAE), and IC50 of 44 ± 4 μg/mL.

Conclusion: The findings of this study suggest that DCM extract may possess anticancer effect against lung adenocarcinoma and brain glioblastoma, as well as serve as an anti-inflammatory agent.

Type de document:Article publié dans une revue avec comité d'évaluation
ISSN:1596-5996
Volume:15
Numéro:4
Pages:p. 751-757
Version évaluée par les pairs:Oui
Date:2016
Identifiant unique:10.4314/tjpr.v15i4.12
Sujets:Sciences naturelles et génie > Sciences naturelles > Biologie et autres sciences connexes
Sciences naturelles et génie > Sciences naturelles > Chimie
Sciences de la santé > Sciences médicales > Pharmacologie
Département, module, service et unité de recherche:Départements et modules > Département des sciences fondamentales
Unités de recherche > Centre de recherche sur la Boréalie (CREB)
Mots-clés:anticancer, bioassay, biological activity, brain glioblastoma, Galega officinalis L, Goat’s rue, lung adenocarcinoma
Déposé le:07 juin 2023 14:06
Dernière modification:07 juin 2023 14:06
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