Karakas Fatma Pehlivan, Turker Arzu Ucar, Karakas Alper et Mshvildadze Vakhtang. (2016). Cytotoxic, anti-inflammatory and antioxidant activities of four different extracts of Galega officinalis L (Goat’s rue). Tropical Journal of Pharmaceutical Research, 15, (4), p. 751-757.
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URL officielle: http://dx.doi.org/doi:10.4314/tjpr.v15i4.12
Résumé
Purpose: To evaluate the cytotoxic, anti-inflammatory and antioxidant activities of four different solvent extracts obtained from the aerial parts of Galega officinalis L.
Methods: The hexane, DCM, methanol and water extracts of G. officinalis were successively obtained by soxhlet extraction method. The cytotoxic activity of the extracts was assessed against human lung carcinoma (A-549), human colorectal adenocarcinoma (HT-29), human brain glioblastoma (U-87), and colon adenocarcinoma (DLD-1) by Resazurine test. The antioxidant activity of extracts were determined by Folin-Ciocalteau, oxygen radical absorbing capacity (ORAC), and 2’.7’-dichlorofluorescin-diacetate (DCFH-DA) cell-based assay while their anti-inflammatory activity was determined by nitric oxide (NO) assay.
Results: DCM extract showed strong cytotoxic activity against lung adenocarcinoma and brain glioblastoma cell lines, with IC50 (concentration inhibiting 50 % of cell growth) values of 11 ± 0.4 and 16 ± 3 μg/mL, respectively. The hexane extract showed moderate anticancer activity against the same cell lines (59 ± 13 and 63 ± 16 μg/mL, respectively). DCM extract also showed significant anti-inflammatory activity, inhibiting NO release by 86.7 % at 40 μg/mL in lipopolysaccharide (LPS) - stimulated murine RAW 264.7 macrophages. Of all test extracts, the methanol extract of G. officinalis showed the highest antioxidant activity with 2.33 ± 0.09 μmol Trolox/mg, 7.10 ± 0.9 g tannic acid equivalent (TAE), and IC50 of 44 ± 4 μg/mL.
Conclusion: The findings of this study suggest that DCM extract may possess anticancer effect against lung adenocarcinoma and brain glioblastoma, as well as serve as an anti-inflammatory agent.
Type de document: | Article publié dans une revue avec comité d'évaluation |
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ISSN: | 1596-5996 |
Volume: | 15 |
Numéro: | 4 |
Pages: | p. 751-757 |
Version évaluée par les pairs: | Oui |
Date: | 2016 |
Identifiant unique: | 10.4314/tjpr.v15i4.12 |
Sujets: | Sciences naturelles et génie > Sciences naturelles > Biologie et autres sciences connexes Sciences naturelles et génie > Sciences naturelles > Chimie Sciences de la santé > Sciences médicales > Pharmacologie |
Département, module, service et unité de recherche: | Départements et modules > Département des sciences fondamentales Unités de recherche > Centre de recherche sur la Boréalie (CREB) |
Mots-clés: | anticancer, bioassay, biological activity, brain glioblastoma, Galega officinalis L, Goat’s rue, lung adenocarcinoma |
Déposé le: | 07 juin 2023 14:06 |
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Dernière modification: | 07 juin 2023 14:06 |
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